Regulatory

Part:BBa_J119129

Designed by: MWSU Genetics Lab Fall 2012 Tues 8:00   Group: Eckdahl Lab   (2013-01-07)


pTac with mutations

This part was designed to see the effects of mutations on a functional promoter. Golden Gate Assembly was used to insert the mutations into Part J100091 at the double terminator sites. A mutation was created by removing the ATTA sequence after the -35 location. This had the effect of reducing the spacing between the -35 and -10 elements from 16 base pairs to 12 base pairs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None